Evaluation of the phenotype pattern of macrophages isolated from malignant and non-malignant pleural effusions

• DOI: 10.1007/s13277-011-0214-1
• Publication Date: 2011-08-01T09:50:32Z
• Authors: Mariusz Kaczmarek; Agata Nowicka; Magdalena Kozłowska; Jakub Żurawski; Halina Batura-Gab...; Jan Sikora
• Keywords: CD163; MMP2

Macrophages are among the infiltrate components of most malignant tumors. Tumor-associated macrophages (TAMs) may secrete a variety humoral factors, which promote or inhibit tumor growth. In general, depending on their activation pathway, exhibit two different patterns phenotype, M1 M2. It is assumed that TAMs comprise pattern pleural effusion, frequent component cytological evaluation. this microenvironment, could be involved in development immunity. The phenotype represented and non-malignant effusions unknown. study, were isolated from 38 (15 23 non-malignant) expression immune mediators receptors was assessed to determine type (M1 vs. M2). mRNA analyzed for IL-1β, IL-4, IL-6, IL-10, IL-11, IL-18, TNFα, TGFβ1, IL1R1, IL1RAP, TLR2, TLR4, VLA4, CD62L, MMP2, MMP9, VEGFA, PDGFA, PDGFB. immunohistochemical evaluation, expressions CD68, mesothelin, MAC387, IL-12, CD105 assessed. cytoplasmic IFNγ, IL-10 surface CD11a, CD14, CD15, CD16, CD23, CD25, CD45, CD54, CD69, VLA2, VLA3, VLA6, CCR7 tested using flow cytometry. supernatants cultures, IL-8, MCP1, VEGF investigated by cytometric beads array method (CBA flex sets) TGFβ1 ELISA. Our results indicate differ each other suggest show comparable while those express similarity M2 but they have not shown classical pattern.