The level of circulating miRNA-10b and miRNA-373 in detecting lymph node metastasis of breast cancer: potential biomarkers

• DOI: 10.1007/s13277-012-0570-5
• Publication Date: 2012-12-13T01:24:28Z
• Authors: Weijie Chen; Fengfeng Cai; Bei Zhang; Zeinab Barekati; Xiao Yan Zhong
• Keywords: Lymphovascular invasion

MicroRNAs (miRNAs) are a class of small noncoding RNAs whose expression changes associated with cancer development and invasion. We hypothesized that miR-10b miR-373, which increased in lymphatic metastatic tissues, could be directly assayed the plasma used to detect lymph node status breast patients. Between November 2009 January 2012, 35 ductal carcinoma patients metastasis (N patients), 25 without (N(0) ten healthy female donors were enrolled study. Circulating miR-373 determined preoperative samples by reverse transcription quantitative real-time PCR assay. In preliminary tests, levels circulating found significantly higher compared N(0) normal (P < 0.01). On validation analysis, median value level N was 4.44-fold 0.01) increased, 4.38-fold comparison MiR-10b for differentiation from patients; odds ratio 2.19, area under receiver-operating curve (AUC) 0.80, sensitivity 71 % specificity 72 %. For 2.62, AUC 0.84, 68 89 A combination two miRNAs further enhanced 94.3 Our data suggest miRNA-10b miRNA-373 potential biomarkers detecting cancer.