Effects of CXCR4 gene silencing by lentivirus shRNA on proliferation of the EC9706 human esophageal carcinoma cell line

• DOI: 10.1007/s13277-013-0858-0
• Publication Date: 2013-06-06T04:07:21Z
• Authors: Dao-feng Wang; Ning Lou; Miao-zhen Qiu; Yong-bin Lin; Ying Liang
• Keywords: Mice, Inbred BALB C; Esophageal Neoplasms; Cell Cycle; Lentivirus; Gene Expression; Mice, Nude; Chemokine CXCL12; 3. Good health; Mice; 03 medical and health sciences; 0302 clinical medicine; Proto-Oncogene Proteins c-bcl-2; Cell Line, Tumor; Gene Knockdown Techniques; Carcinoma, Squamous Cell; Animals; Humans; Female; RNA Interference; RNA, Messenger; RNA, Small Interfering; Neoplasm Transplantation; Cell Proliferation

CXCL12/CXCR4 has been studied as an important biomarker for many human malignancies, but studies are limited esophageal squamous cell carcinoma (ESCC). In this study, effective RNAi sequence targeting the CXCR4 gene was selected, a lentiviral shRNA vector constructed to specifically silence expression in EC9706 ESCC line, and effects of silencing on growth vitro tumour nude mice were then evaluated. The significantly downregulated after transfection with vector. apoptosis-related Bcl-2 decreased. addition, inhibition, considerably inhibited, increased apoptosis cells found, G0/G1 percentage increased, number S phase reduced. Moreover, inhibited. conclusion, downregulation by could inhibit proliferation. Our data may provide avenue finding new treatments.