MicroRNA-455 inhibits proliferation and invasion of colorectal cancer by targeting RAF proto-oncogene serine/threonine-protein kinase

• DOI: 10.1007/s13277-014-2766-3
• Publication Date: 2014-10-29T16:37:30Z
• Authors: Jie Chai; Shan Wang; Dali Han; Wei Dong; Chao Xie; Hongliang Guo
• Keywords: AKT2; AKT3; c-Raf

Colorectal cancer (CRC, also known as colon cancer, rectal or bowel cancer) is the second leading cause of mortality in Western world. MicroRNAs (miRNAs) are a class small (18-25 nucleotides long) noncoding RNAs with important posttranscriptional regulatory functions. miRNAs play roles various physiological and pathological processes including carcinogenesis solid cancers CRC. In order to investigate that played CRC, expression human (in 20 normal adjacent tissue samples samples) was examined this study. miR-455, miR-484, miR-101 were significantly downregulated samples. And overexpression miR-455 inhibited proliferation invasion SW480, but had no effect on apoptosis. PCR blot showed decreased protein RAF proto-oncogene serine/threonine-protein kinase (RAF1) mRNA level. Luciferase assay indicated regulated RAF1 directly. Moreover, partially reversed inhibitory growth SW480. The data regulates colorectal cells, at least part, by downregulating RAF1, direct target miR-455. Collectively, our study demonstrated miR-455-RAF1 may represent new potential therapeutic for carcinoma treatment.