Long noncoding RNA SPRY4-IT1 predicts poor patient prognosis and promotes tumorigenesis in gastric cancer

• DOI: 10.1007/s13277-015-3376-4
• Publication Date: 2015-04-03T11:16:39Z
• Authors: Wei Peng; Guoqiu Wu; Hong Fan; Jianzhong Wu; Jifeng Feng
• Keywords: Adult; Male; Carcinogenesis; Middle Aged; Prognosis; Disease-Free Survival; 3. Good health; Gene Expression Regulation, Neoplastic; 03 medical and health sciences; 0302 clinical medicine; Cell Movement; Stomach Neoplasms; Cell Line, Tumor; Biomarkers, Tumor; Humans; Female; Neoplasm Invasiveness; RNA, Long Noncoding; Aged

Gastric cancer (GC) is the second common cause of cancer-related death worldwide. Long noncoding RNAs (lncRNAs) are emerging as novel regulators in paradigm. However, investigation lncRNAs on GC still its infancy. In this study, we focused lncRNA SPRY4 intronic transcript 1 (SPRY4-IT1) and investigated expression pattern, clinical significance, biological function, molecular mechanism GC. SPRY4-IT1 was examined, correlation with clinicopathological characteristics patient prognosis analyzed. A series assays were performed to understand role elevated tissues cell lines, levels highly positively correlated tumor size, invasion depth, distant metastasis, TNM stage, reduced overall survival (OS) disease-free (DFS). multivariate analysis showed that an independent prognostic factor OS DFS patients Additionally, results vitro suppression line MKN-45 significantly proliferation, colony formation, migration/invasion. Moreover, tumorigenic effects partially mediated by regulation certain cyclins matrix metalloproteinases (MMPs)-related genes. Our data suggest plays a critical tumorigenesis may represent marker potential therapeutic target