MicroRNA-199a-3p suppresses glioma cell proliferation by regulating the AKT/mTOR signaling pathway

• DOI: 10.1007/s13277-015-3409-z
• Publication Date: 2015-04-08T05:47:31Z
• Authors: Liang Shen; Chunming Sun; Yanyan Li; Xuetao Li; Ting Sun; Chuanjin Liu; Youxin Zhou; Ziwei Du
• Keywords: Male; Cancer Research; 0303 health sciences; TOR Serine-Threonine Kinases; Apoptosis; Glioma; Prognosis; Gene Expression Regulation, Neoplastic; MicroRNAs; 03 medical and health sciences; Cell Line, Tumor; Humans; Female; Research Article; Cell Proliferation

Glioma has been investigated for decades, but the prognosis remains poor because of rapid proliferation, its aggressive potential, and resistance to chemotherapy or radiotherapy. The mammalian target rapamycin (mTOR) is highly expressed regulates cellular proliferation cell growth. MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene transcription translation via up-regulating down-regulating levels miRNAs. This study was conducted explore molecular functions miR-199a-3p in glioma. We detected expression glioma samples by quantitative PCR (qPCR). Then, we transfected U87 U251 lines with miR-199a-3p. Cellular invasion, apoptosis were assessed explain function confirmed lower combined normal brain tissues. over-expression might mTOR restrained growth not invasive capability. Results indicated inhibited AKT/mTOR signaling pathway elevating MiR-199a-3p effects similar tumor suppressor on pathway.