Exosomes from adriamycin-resistant breast cancer cells transmit drug resistance partly by delivering miR-222

• DOI: 10.1007/s13277-015-4161-0
• Publication Date: 2015-10-02T18:40:06Z
• Authors: Dan-dan Yu; Ying Wu; Xiao-hui Zhang; Meng-meng Lv; Wei-xian Chen; Xiu Chen; Su-jin Yang; Hongyu Shen; Shan-liang Zhong; Jin-hai Tang; Jian-hua Zhao
• Keywords: MCF-7; MTT assay; Viability assay

Breast cancer (BCa) is one of the major deadly cancers in women. However, treatment BCa still hindered by acquired-drug resistance. It increasingly reported that exosomes take part development, metastasis, and drug resistance BCa. specific role poorly understood. In this study, we investigate whether transmit through delivering miR-222. We established an adriamycin-resistant variant Michigan Cancer Foundation-7 (MCF-7) breast cell line (MCF-7/Adr) from a drug-sensitive (MCF-7/S). Exosomes were isolated supernatant ultracentrifugation. Cell viability was assessed MTT assay apoptosis assay. Individual miR-222 molecules cells detected fluorescence situ hybridization (FISH). Then, FISH combined with locked nucleic acid probes enzyme-labeled (LNA-ELF-FISH). could be as bright photostable fluorescent spots then quantity per counted. Stained taken receipt cells. MCF-7/S acquired after co-culture MCF-7/Adr (A/exo) but did not (S/exo). The A/exo-treated significantly greater than S/exo-treated MCF-7/S. transfected mimics adriamycin while inhibitors lost conclusion, are effective transmitting delivery via may mechanism.