The role of miR-145 in stem cell characteristics of human laryngeal squamous cell carcinoma Hep-2 cells

• DOI: 10.1007/s13277-015-4219-z
• Publication Date: 2015-10-21T21:15:53Z
• Authors: Omer Faruk Karatas; Ilknur Suer; Betul Yuceturk; Mehmet Yilmaz; Yusif Hajiyev; Chad J. Creighton; Michael Ittmann; Mustafa Ozen
• Keywords: KLF4; Stem cell marker; Homeobox protein NANOG

The cancer stem-like cells (CSLCs) are tumorigenic promoting initiation, progression, and spread of the tumor. Accumulating evidences suggested presence CSLCs in distinct tumors including laryngeal squamous cell carcinoma (LSCC). MicroRNAs have been proposed as significant regulators carcinogenesis, several them demonstrated to direct roles survival CSLCs. In this study, we aimed explore role miR-145, which is downregulated LSCC, on stem potency cells. We initially showed downregulation miR-145 expression tumor tissue samples CD133-enriched Quantitative reverse-transcription PCR (qRT-PCR) analysis miR-145-transfected Hep-2 inhibitory markers like SOX2, OCT4, KLF4, ABCG2. We, then, investigated features miR-145-overexpressing by sphere formation assay, single-cell cloning aldehyde dehydrogenase (ALDH) all inhibition upon overexpression. Further qRT-PCR altered epithelial mesenchymal transition conclusion, regulatory characteristics Based these results, propose that might carry crucial LSCC tumorigenesis, prognosis, metastasis, chemoresistance, recurrence through regulating properties