Effects of silibinin on growth and invasive properties of human ovarian carcinoma cells through suppression of heregulin/HER3 pathway

• DOI: 10.1007/s13277-015-4220-6
• Publication Date: 2015-10-19T03:11:50Z
• Authors: Majid Momeny; Reza Ghasemi; Giovanni Valenti; Mariska Miranda; Ali Zekri; Ghazaleh Zarrinrad; Sepehr Javadikoos...; Marjan Yaghmaie; Kamran Alimoghaddam; Ardeshir Ghavamzadeh; Seyed H. Ghaffari
• Keywords: Silibinin; neuregulin 1

Epithelial ovarian cancer (EOC) is the most fatal gynecological malignancy due to its high proliferative and invasive capacities. A heregulin (HRG)/HER3 autocrine loop increases metastatic properties of EOC cells, suggesting that modulators this signaling pathway may prove effective trammel growth motility these cells. This study aimed evaluate effects multi-tyrosine kinase inhibitor silibinin on characteristics cell lines OVCAR8 SKOV3 through suppression HRG/HER3 pathway. To achieve this, proliferation, DNA synthesis, clonogenicity, cycle progression, cathepsin B enzymatic activity, migration invasion were explored in vitro. Silibinin suppressed clonogenic abilities cells inhibition circuit. Silibinin-mediated attenuation HER3 disabled HER3/AKT/survivin axis thereby, induced G1/S arrest. Furthermore, reduced potentials quelling activity. Altogether, results suggest a potential anti-cancer drug inhibit exhibit an