In vitro and in vivo anti-melanoma effects of Daphne gnidium aqueous extract via activation of the immune system

• DOI: 10.1007/s13277-015-4492-x
• Publication Date: 2015-12-03T23:07:58Z
• Authors: Fadwa Chaabane; Nadia Mustapha; Imen Mokdad-Bzeouich; Aicha Sassi; Soumaya Kilani-Ja...; Marie-Geneviève D...; Serge Michalet; Mayssa Fathallah; Mounira Krifa; Kamel Ghedira; Leila Chekir-Ghedira
• Keywords: Splenocyte; CTL*; MTT assay

The purpose of this study was to assess the antitumor and immunomodulatory effects aqueous extract from Daphne gnidium in mice-bearing melanoma tumor. Balb/C mice were subcutaneously implanted with B16-F10 cells treated intraperitoneally at 200 mg/Kg b.w for 21 days. After euthanization on day 22, tumors weighed; lymphocyte proliferation, cytotoxic T (CTL), natural killer (NK) cell activities evaluated using MTT assay. Macrophage phagocytosis studied by measuring lysosomal activity. In addition its potential inhibit growth transplantable tumor, remarkably induced splenocyte proliferation both NK CTL tumor-bearing mice. also seen have promoted activity host macrophages.