Silencing of HMGA2 suppresses cellular proliferation, migration, invasion, and epithelial–mesenchymal transition in bladder cancer

• DOI: 10.1007/s13277-015-4625-2
• Publication Date: 2015-12-18T05:50:48Z
• Authors: Zhan Shi; Xiang Li; Ding Wu; Run Tang; Renfu Chen; Song Xue; Xiaoqing Sun
• Keywords: HMGA2; MMP2

The high-mobility group protein A2 (HMGA2) is an architectural transcription factor that plays a crucial role in the development and progression of various malignant cancers. However, function HMGA2 bladder cancer remains largely unknown. Therefore, we aim to investigate effect on proliferation, migration, invasion, epithelial-mesenchymal transition (EMT) cells. expression human cells was downregulated by small interfering RNA (siRNA). levels other related proteins were detected Western blotting. cell proliferation apoptosis examined Cell Counting Kit-8 flow cytometry, respectively. Transwell migration invasion assays performed assess ability In conclusion, found knockdown markedly inhibited proliferation; this reduced growth due high rate cells, as Bcl-xl diminished, whereas Bax upregulated. Moreover, our results showed silencing greatly decreased matrix metalloproteinase-2 (MMP-2) metalloproteinase-9 (MMP-9), affected occurrence EMT. We further suppressed transforming factor-β (TGF-β)/Smad Wnt/β-catenin signaling pathway These revealed played important might be novel target for therapy cancer.