Hyperthermotherapy enhances antitumor effect of 5-aminolevulinic acid-mediated sonodynamic therapy with activation of caspase-dependent apoptotic pathway in human glioma

• DOI: 10.1007/s13277-016-4931-3
• Publication Date: 2016-02-04T14:11:59Z
• Authors: Donghui Ju; Fumio Yamaguchi; Guangzhi Zhan; Tadashi Higuchi; Takayuki Asakura; Akio Morita; Hideo Orimo; Shaoshan Hu
• Keywords: Sonodynamic therapy; Viability assay

Sonodynamic therapy (SDT) has shown great potential as an approach for cancer treatment, and hyperthermotherapy (HT) is also a promising therapy. Here, we investigate whether HT could improve the efficacy of SDT to make preliminary exploration on mechanism. Xenograft tumor was established in nude mice model, SNB19 U87MG glioma cell lines were utilized vitro experiment. Alamar blue assay performed assess viability. Optical microscope used characterize morphology changes cells induced by treatments. Apoptotic rate, mitochondrial membrane (MMP), intracellular production reactive oxygen species (ROS) examined flow cytometer. The apoptosis tissues detected TUNEL assay. Furthermore, expression apoptosis-related proteins with Western blot immunohistochemistry vivo. plus group significantly reduce viability circular-cell morphological change, compared group, decreased depending raise 5-ALA concentration, ultrasound exposure time, temperature. results indicate that increased conspicuous apoptosis, ROS production, remarkable loss MMP 5-ALA-SDT vitro. Meanwhile, our data demonstrated combined treatment induce delay growth both vivo experiments, expressed higher protein levels Bax cleaved caspase-3, 8, 9 SDT, HT, control groups lower level bcl-2 than other three groups, while these unchanged between groups. may provide important promotion further propose combination new application human glioma.