Creation of an apoptin-derived peptide that interacts with SH3 domains and inhibits glioma cell migration and invasion

• DOI: 10.1007/s13277-016-5404-4
• Publication Date: 2016-09-29T06:12:50Z
• Authors: Weiwei Song; Hengyu Zhao; Zhongqi Cui; Xiaoyu Ma; Wenwen Zhang; Di Wang; Anqi Liu; Lijie Yuan
• Keywords: Viability assay; MTT assay

Glioblastoma multiforme (GBM) is an aggressive tumor of the central nervous system characterized by high rates recurrence, morbidity, and mortality. This study investigated antitumor effects apoptin-derived peptide (ADP) on glioma cells explored underlying mechanisms. The U251, U87, C6 cell lines were used in present study, expression p-Akt, Akt, MMP-9 was determined through Western blotting, quantitative real-time PCR, hematoxylin eosin (HE) staining. Tumor growth evaluated magnetic resonance imaging, viability assessed 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide MTT assay. Glioma metastasis using transwell migration, invasion, scratch-wound assays. An ADP designed synthesized based results a domain-based analysis structure apoptin. inhibited viability, invasion treatment with led to downregulation p-Akt translation. also migration vivo, HE staining showed decreases satellite-like masses apoptotic populations after ADP. Our findings demonstrate that can suppress via PI3K/Akt/MMP-9 signaling pathway provide new platform for development drugs treating glioma.