Exploring unsaturated fatty acid cholesteryl esters as transdermal permeation enhancers

• DOI: 10.1007/s13346-017-0360-0
• Publication Date: 2017-02-03T09:53:42Z
• Authors: Sanjeev Rambharose; Rahul S. Kalhapure; Mahantesh Jadhav; Thirumala Govender
• Keywords: Male; Skin Absorption; Fatty Acids; In Vitro Techniques; Administration, Cutaneous; 3. Good health; 03 medical and health sciences; 0302 clinical medicine; Animals; Cholesterol Esters; Rats, Wistar; Tenofovir; Skin

The intrinsic protective barrier property of skin, one of the major challenges in the design of transdermal drug delivery systems, can be overcome through the use of chemical permeation enhancers (CPEs). Herein, we explore the potential of unsaturated fatty acid (UFA) esters of cholesterol (Chol) viz., oleate, linoleate and linolenate, as transdermal CPEs using tenofovir (TNF) as a model drug. All Chol UFA esters at 1% w/w were found to be more effective enhancers when compared to their respective parent fatty acids (FAs) and saturated FA counterparts. Cholesteryl linolenate (Chol-LLA) showed the most superior performance (enhancement ratio (ER) = 3.71). The greatest ER for Chol-LLA (5.93) was achieved at a concentration of 2% w/w. The histomorphological and transepithelial electrical resistance (TEER) evaluations supported the results of the permeability studies. These findings showed no significant loss in the integrity of the epidermis, with drug and enhancer treatment having temporary effects on the barrier property of the epidermis. Chol UFA esters can therefore be considered as new CPEs for exploitation in topical formulations for various classes of drugs.