Polymorphism of mycotoxin biosynthetic genes among Fusarium equiseti isolates from Italy and Poland

• DOI: 10.1007/s13353-012-0085-1
• Publication Date: 2012-02-21T10:01:34Z
• Authors: Łukasz Stępień; Karolina Gromadzka; Jerzy Chełkowski
• Keywords: 2. Zero hunger; 0303 health sciences; Polymorphism, Genetic; Genes, Fungal; Mycotoxins; Phylogeography; 03 medical and health sciences; Peptide Elongation Factor 1; Fusarium; Italy; Depsipeptides; Microbial Genetics · Original Paper; Genetics; Zearalenone; Poland; Trichothecenes; Polyketide Synthases; Phylogeny

Fusarium equiseti (Corda) Saccardo is a soil saprophyte and weak pathogen, associated with several diseases of fruit other crops in subtropical tropical areas, but also countries temperate climate. A wide range secondary metabolites has been identified among natural F. populations, zearalenone (ZEA), fusarochromanone fusarenon-X being the most common. In present study, genetic diversity strains from two populations (from Italy Poland) was evaluated by analysing translation elongation factor 1α (tef-1α) sequences, polyketide synthases ZEA biosynthetic pathway (PKS13 PKS4) TRI5 gene trichothecene pathway. produced rice cultures 20 27 tested isolates concentrations ranging 1.34 ng/g to 34,000 ng/g). The ability produce enniatins trichothecenes all identifying esyn1, TRI13 TRI4 genes. presence PKS4 PKS13 genes confirmed polymerase chain reaction (PCR) only some ZEA-producing isolates. Similarly, found 14 tested. This likely have caused divergence those between graminearum (the latter species used for primers design) can be exploited phylogenetic studies. analysis mycotoxin sequences differentiate studied genotypes even more precisely than non-coding regions (like tef-1α).