Laserspray and Matrix-Assisted Ionization Inlet Coupled to High-Field FT-ICR Mass Spectrometry for Peptide and Protein Analysis

• DOI: 10.1007/s13361-012-0545-1
• Publication Date: 2013-02-04T20:12:11Z
• Authors: Leonard Nyadong; Ellen D. Inutan; Xu Wang; Christopher L. He...; Sarah Trimpin; Alan G. Marshall
• Keywords: Matrix (chemical analysis)

We present the first coupling of laser spray ionization inlet (LSII) and matrix assisted (MAII) to high-field Fourier transform ion cyclotron resonance mass spectrometry (FT-ICR MS) for generation electrospray-like ions take advantage increased sensitivity, range, resolving power afforded by multiple charging. apply technique top-down protein analysis characterization metalloproteins. also a novel method multiply-charged copper-peptide complexes with varying degrees copper adduction LSII. show an application generated charge state molecular weight determination, particularly useful instrument such as linear trap analyzer.