Analysis of the Lipidome of Xenografts Using MALDI-IMS and UHPLC-ESI-QTOF

• DOI: 10.1007/s13361-014-0882-3
• Publication Date: 2014-04-23T14:22:35Z
• Authors: Roberto Fernández; Sergio Lage; Beatriz Abad-García; Gwendolyn Barceló...; Silvia Terés; Daniel H. López; Francisca Guardio...; M. Laura Martín; Pablo V. Escribá; José A. Fernández
• Keywords: Lipidome

Human tumor xenografts in immunodeficient mice are a very popular model to study the development of cancer and test new drug candidates. Among parameters analyzed variations lipid composition, as they good indicators changes cellular metabolism. Here, we present on distribution lipids NCI-H1975 human lung cells, using MALDI imaging mass spectrometry UHPLC-ESI-QTOF. The identification directly from tissue by was aided comparison with ESI ionization extracts same xenografts. Lipids belonging PCs, PIs, SMs, DAG, TAG, PS, PA, PG classes were identified their over xenograft determined. Three areas xenograft, corresponding cells different metabolic stages layer adipose that covers xenograft.