Extensive Charge Reduction and Dissociation of Intact Protein Complexes Following Electron Transfer on a Quadrupole-Ion Mobility-Time-of-Flight MS

• DOI: 10.1007/s13361-015-1124-z
• Publication Date: 2015-04-11T00:20:01Z
• Authors: Frederik Lermyte; Jonathan P. Williams; Jeffery M. Brown; Esther M. Martin; Frank Sobott
• Keywords: Electron-transfer dissociation; Ion-mobility spectrometry; Time-of-Flight; Collision-induced dissociation

Non-dissociative charge reduction, typically considered to be an unwanted side reaction in electron transfer dissociation (ETD) experiments, can enhanced significantly order reduce the state of intact protein complexes as low 1+ on a commercially available Q-IM-TOF instrument. This allows for detection large beyond 100,000 m/z, while at same time generating top-down ETD fragments, which provide sequence information from surface-exposed parts folded structure. Optimization supplemental activation has proven crucial these experiments and charge-reduced species are most likely product both proton (PTR) non-dissociative (ETnoD) reactions that occur prior ion mobility cell. Applications this approach range deconvolution complex spectra manipulation states gas-phase ions.