Rapid and High-Throughput Detection and Quantitation of Radiation Biomarkers in Human and Nonhuman Primates by Differential Mobility Spectrometry-Mass Spectrometry

• DOI: 10.1007/s13361-016-1438-5
• Publication Date: 2016-07-08T14:10:03Z
• Authors: Zhidan Chen; Stephen L. Coy; Evan L. Pannkuk; Evagelia C. Laiakis; Adam B. Hall; Albert J. Fornace; Paul Vouros
• Keywords: Ion-mobility spectrometry; Ion-mobility spectrometry–mass spectrometry

Radiation exposure is an important public health issue due to a range of accidental and intentional threats. Prompt effective large-scale screening appropriate use medical countermeasures (MCM) mitigate radiation injury requires rapid methods for determining the dose. In number studies, metabolomics has identified small-molecule biomarkers responding Differential mobility spectrometry-mass spectrometry (DMS-MS) been used similar compounds high-throughput detection quantitation. this study, we show that DMS-MS can detect quantify two biomarkers, trimethyl-L-lysine (TML) hypoxanthine. Hypoxanthine human nonhuman primate (NHP) biomarker metabolic intermediate, whereas TML in humans but not NHP, which involved carnitine synthesis. They have analyzed by from urine samples after simple strong cation exchange-solid phase extraction (SCX-SPE). The dramatic suppression background chemical noise provided results approximately 10-fold reduction time, including sample pretreatment compared with liquid chromatography-mass (LC-MS). quantitation accuracy verified validation testing each biomarker. Human are yet available, hypoxanthine, selected NHP (pre- 7-d-post 10 Gy exposure) were analyzed, resulting mean change concentration essentially identical obtained LC-MS (fold-change 2.76 versus 2.59). These confirm potential field or clinical first-level exposure.