Presence of regulatory T-cells in endometrial cancer predicts poorer overall survival and promotes progression of tumor cells

• DOI: 10.1007/s13402-022-00708-2
• Publication Date: 2022-09-13T15:11:57Z
• Authors: Thomas Kolben; Mareike Mannewitz; Carolin Perleberg; Konstantin Schnell; David Anz; Laura Hahn; Sarah Meister; Elisa Schmoeckel; Alexander Burges; Bastian Czogalla; Anna Hester; Sven Mahner; Mirjana Kessler; Udo Jeschke; Stefanie Corradini; Fabian Trillsch; Susanne Beyer
• Keywords: Regulatory T cell; Tumor progression

Abstract Purpose Endometrial cancer (EC) is one of the most common gynaecologic malignancies. Tumor infiltrating regulatory T-cells (Treg) have been reported to a prognostic impact in many Immunotherapeutic strategies are gaining interest for advanced and recurrent EC cases, where treatment options rare. Our study was aimed at determining value Treg progression. Methods specimens from 275 patients 28 controls were screened immunohistochemically presence represented by FoxP3. Correlations with clinicopathological survival parameters performed. Functional assays performed using cell lines Ishikawa + RL95-2 after co-culturing isolated CD4 CD25 CD127dim Treg. To assess influence on composition peripheral blood mononuclear cells (PBMC), flow cytometric analyses Results We found that an increased infiltration associated high grades reduced overall survival. almost absent endometrium tissues healthy control patients. Co-culture tumor led functional changes: enhanced invasion, migration viability indicated levels microenvironment may promote growth. Furthermore, we phenotypic changes PBMC, showing significantly Conclusion results indicate environment poorer outcome. A remarkable behaviour vice versa PBMC subpopulations support this notion mechanistically. findings provide basis focusing as potential future therapeutic targets EC.