Effect of temperature shifts and anammox biomass immobilization on sequencing batch reactor performance and bacterial genes abundance

• DOI: 10.1007/s13762-020-02957-w
• Publication Date: 2020-10-10T07:03:07Z
• Authors: A. Banach-Wiśniewska; M. Ćwiertniewicz-...; A. Ziembińska-Buc...
• Keywords: Anammox; Sequencing batch reactor

Abstract Implementation of anaerobic ammonium oxidation (anammox) below its optimal temperature, known as “cold anammox”, may lead to common use in wastewater treatment plants, reducing the operational costs treatment. Thus, we investigated effects immobilization polyvinyl alcohol–sodium alginate gel beads on anammox performance at temperatures 30 °C, 23 and 15 °C laboratory-scale sequencing batch reactors. We determined relative gene abundance nitrogen removal bacterial groups, which are considered key functional microbes cycle activated sludge: denitrifies, ammonia-oxidizing bacteria, nitrite-oxidizing bacteria. Nitrogen efficiency was higher for immobilized sludge comparison with non-immobilized biomass each temperature. At 83.7 ± 6.46% reactor, 79.4 7.83% control while remained level 50 2.5% fluctuated from 13.2 45.3% one. During temperature shifts, process also more stable case reactor biomass. A statistically significant correlation found between hydrazine oxidoreductase abundance.