The mixture of glycitin and TDB (3-(2,4,6-trimethoxyphenyl)-2,3-dihydro-1H-benzo[f]chromen-1-one) could ameliorate skin ageing by anti-wrinkle and anti-melanogenesis effects in dermal fibroblasts and melanocytes

• DOI: 10.1007/s13765-014-4304-9
• Publication Date: 2014-12-02T07:34:20Z
• Authors: Manh Tin Ho; Dongsoo Koh; Moonjae Cho
• Keywords: Dermal fibroblast; Melanocyte; Wrinkle; Human skin

Ultraviolet (UV) radiation in sunlight can induce skin ageing and photo-carcinogenesis. UV may also induce melanin production and wrinkle formation. Recently, natural secondary compounds have been reported to have protective effects against UV light. In this study, the effects of two different compounds, glycitin and 3-(2,4,6-trimethoxyphenyl)-2,3-dihydro-1H-benzo[f]chromen-1-one (TDB), on human dermal fibroblasts and melanocytes were investigated. At first, only TDB was used on melanocyte cells to test whether it inhibited the proliferation of these cells. Then, a mixture of glycitin and TDB was tested on human dermal fibroblasts for 48 h in order to investigate its effect on proliferation, collagen production, and metalloproteinase family expression. The TDB treatment alone not only inhibited the proliferation of melanocytes but also increased extra cellular matrix production in dermal fibroblasts and cell viability. The mixture of glycitin and TDB markedly increased fibroblast proliferation and helped to maintain fibroblast viability in the face of UV-induced and H2O2-induced damages. This co-treatment also significantly promoted collagen IV expression and accelerated total collagen secretion. In addition, the metalloproteinase (MMPs) family such as MMP1, MMP2, and MMP7 were down-regulated at the transcriptional level. In conclusion, the mixture of glycitin and TDB induced fibroblast proliferation even when these fibroblasts were damaged by UV exposure and H2O2, whereas augmented collagen production and the inhibition of MMPs reduced wrinkle formation and decreased melanocyte proliferation, suggesting a potential use in UV-protective therapy.