Validation protocol for whole-body dosimetry in an agricultural exposure study

• DOI: 10.1007/s13765-017-0330-8
• Publication Date: 2017-11-17T01:29:02Z
• Authors: Jiho Lee; Eunhye Kim; Yongho Shin; Jonghwa Lee; Junghak Lee; Wolfgang Maasfeld; Jeong-Han Kim
• Keywords: Repeatability

Agricultural workers exposed to pesticides can experience adverse health impacts depending on toxicity and exposure amount. Whole-body dosimetry (WBD) is the most reliable, practical, realistic method for measuring exposure. Since validation of analytical experimental methodologies critical quantitative determination exposure, we conducted a procedure design an essential protocol WBD studies. Using fungicide kresoxim-methyl, matrix-matched standards were prepared with various matrices including outer cloth, inner washing solution gloves hands, gauze, glass fiber filter (IOM sampler) determine instrumental limit quantitation high-performance liquid chromatography (HPLC) (2 ng) chromatography–tandem mass spectrometry (LC–MS/MS) (10 pg). Method limits (MLOQ) also set HPLC (0.1 mg/L) LC–MS/MS (0.005 mg/L). We observed good analysis repeatability (coefficient variation < 6%), linearity calibration curves was reasonable (r 2 > 0.998) in range 0.001–10 mg/L matrices. Recovery tests carried out at three levels concentration (MLOQ, 10 MLOQ, 100 MLOQ) resulted recoveries (72.7–105.6%). did not observe breakthrough compound holding capacity pesticide filters. The procedures established present study are applicable as essential, comprehensive assessment studies using WBD.