Comparative of fibroblast and osteoblast cells adhesion on surface modified nanofibrous substrates based on polycaprolactone

• DOI: 10.1007/s40204-016-0059-1
• Publication Date: 2016-12-08T08:57:01Z
• Authors: Fereshteh Sharifi; Shiva Irani; Mojgan Zandi; Masoud Soleimani; Seyed Mohammad At...
• Keywords: Polycaprolactone; Electrospinning

One of the determinant factors for successful bioengineering is to achieve appropriate nano-topography and three-dimensional substrate. In this research, polycaprolactone (PCL) nano-fibrous mat with different roughness modified O2 plasma was fabricated via electrospinning. The purpose study evaluate effect modification along surface mats on quality human fibroblast (HDFs) osteoblast cells (OSTs)-substrate interaction. Surface properties were studied using scanning electron microscopy (SEM), atomic force (AFM), contact angle, Fourier-transformation infrared spectroscopy. We evaluated mechanical by tensile test. viability proliferation HDFs OSTs substrates followed 3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT). Mineralization substrate determined alizarin red staining method calcium content kit. Cells morphology SEM analysis. results revealed that plasma-treated electrospun higher an excellent designed A bioactive topography stimulating accelerate latter's differentiation time. Therefore, PCL high density major considered as a bio-functional elegant bio-substrate tissue regeneration applications.