An Analysis of Polymorphisms Within the Wnt Signaling Pathway in Relation to Ovarian Cancer Risk in a Polish Population

• DOI: 10.1007/s40291-013-0059-y
• Publication Date: 2013-09-27T09:56:13Z
• Authors: Adrianna Mostowska; Piotr Pawlik; Stefan Sajdak; Janina Markowska; Monika Pawałowska; Margarita Lianeri; Paweł P. Jagodzinski
• Keywords: AXIN2; SNP; High Resolution Melt; SNP genotyping

The Wnt/β-catenin signaling pathway has been considered to be a factor in the development and progression of ovarian cancer.All patients with cancer controls were tested for BRCA1 mutations (5382incC, C61G, 4153delA) HybProbe assays BRCA2 mutation (5946delT) using high-resolution melting curve analysis (HRM). Mutation carriers excluded from association analysis. We studied nine single nucleotide polymorphisms (SNPs) located CTNNB1 (β-catenin) [rs4533622, rs2953], APC (rs11954856, rs351771, rs459552), AXIN2 (rs4074947, rs7224837, rs3923087, rs2240308) women without BRCA1/BRCA2 (n = 228) 282). Genotyping rs4533622, rs2953, rs4074947, rs2240308 was performed by HRM, while that rs11954856, rs459552 rs7224837 conducted PCR followed appropriate restriction enzyme digestion [PCR–restriction fragment length polymorphism (PCR-RFLP)].The most common identified 30 cancer. These not found controls. lowest p values trend test (p trend) observed rs351771 rs11954856 SNPs 0.006 0.007, respectively). Using dominant inheritance model, we SNP is associated an increased risk [odds ratio 2.034 (95 % CI 1.302–3.178); 0.002]. also significant allelic differences between 0.006).Our study demonstrated significantly frequencies Polish