Development of digital organ-on-a-chip to assess hepatotoxicity and extracellular vesicle-based anti-liver cancer immunotherapy

• DOI: 10.1007/s42242-022-00188-1
• Publication Date: 2022-03-03T12:11:36Z
• Authors: Guohua Wu; Jianguo Wu; Zihan Li; Shengyu Shi; Di Wu; Xuanbo Wang; Han Xu; Hui Liu; Yixiao Huang; Rending Wang; Jia Shen; Zhihong Dong; Shuqi Wang
• Keywords: Liver Cancer

Abstract Organ-on-a-chip systems have been increasingly recognized as attractive platforms to assess toxicity and develop new therapeutic agents. However, current organ-on-a-chip are limited by a “single pot” design, which inevitably requires holistic analysis limits parallel processing. Here, we developed digital combining microwell array with cellular microspheres, significantly increased the parallelism over traditional for drug development. Up 127 uniform liver cancer microspheres in this format served individual analytical units, allowing high consistency quick response. Our platform displayed evident anti-cancer efficacy at concentration of 10 μM sorafenib, had greater alignment than previous vivo study. In addition, demonstrated treatment natural killer cell-derived extracellular vesicles 50 μg/mL. The successful development provides high-parallelism low-variability tool assessment exploration anticancer modalities, thereby accelerating joint endeavor combat cancer. Graphic abstract