Developing Thick Cardiac Tissue with a Multilayer Fiber Sheet for Treating Myocardial Infarction

• DOI: 10.1007/s42765-023-00313-4
• Publication Date: 2023-07-31T08:02:27Z
• Authors: Junjun Li; Xiang Qu; Li Liu; Lingjun Li; Ying Hua; Jingbo Zhang; Masako Ishida; Noriko Yoshida; Akiko Tabata; Nagako Sougawa; Emiko Ito; Noriko Mochizuki-Oda; Akima Harada; Takuji Kawamura; Ryohei Matsuura; Yingzhe Wang; Keisuke Morishima; Shigeru Miyagawa; Yoshiki Sawa
• Keywords: 0303 health sciences; 03 medical and health sciences; 3. Good health

Abstract Human-induced pluripotent stem cell (hiPSC)-derived cardiac patches have been extensively used for treating myocardial infarction and shown potential clinical application. However, the limited patch thickness can hamper its therapeutic effect. We previously developed a fibrous scaffold that allowed formation of well-organized tissue constructs. In present study, based on above technology, we three-dimensional multilayer with dynamic perfusion, which approximately 20 million hiPSC-derived cardiomyocytes (CMs) could be seeded in single step organized into 1 mm thick viable tissue. The demonstrated enhanced contractile properties upregulated cytokine secretion compared control group. Notably, when model, group showed improved functional recovery less fibrosis. These results indicated appropriate hiPSC-CM dose requires careful evaluation developing therapy. significant improvement than group, indicating higher doses transplanted cells may effects infarction.