Decreased biofilm formation in Proteus mirabilis after short-term exposure to a simulated microgravity environment

• DOI: 10.1007/s42770-021-00588-y
• Publication Date: 2021-09-24T04:35:20Z
• Authors: Dapeng Wang; Po Bai; Bin Zhang; Xiaolei Su; Xuege Jiang; Tingzheng Fang; Junfeng Wang; Changting Liu
• Keywords: Weightlessness

Microbes threaten human health in space exploration. Studies have shown that Proteus mirabilis has been found habitats. In addition, the biological characteristics of P. studied unconditionally. The simulated microgravity environment provides a platform for understanding changes mirabilis.This study intends to explore effect on mirabilis, formation biofilm, and its related mechanism.The strange deformable rods were cultured continuously 14 days under high-aspect rotating vessels (HARVs). morphology, growth rate, metabolism, biofilm strain measured, phenotypic evaluated. Transcriptome sequencing was used detect differentially expressed genes compared with phenotype.The metabolic ability, forming ability lower than those normal gravity culture condition microgravity. Further analysis showed decrease may be caused by downregulation (pstS, sodB, fumC).The enables us potential relationship between bacterial phenotype molecular biology, thus opening up suitable constructive method medical fields not explored before. It certain strategy treatment infectious diseases exploring mirabilis.