The Anti-inflammatory and Immune-Boosting Potential of Quercetin-3-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside in LPS–Stimulated RAW264.7 Macrophages

• DOI: 10.1007/s43450-020-00027-3
• Publication Date: 2020-04-09T15:03:46Z
• Authors: Hyun-Gug Jung; Han-Hyuk Kim; Debasish Kumar Dey; Jae Myo Yu; Hyunjeong Kim; Yong-hun Cho; Jae-Yoon Jang; Jae-Yong Park; Sun Chul Kang; Bong Jeun An
• Keywords: Boosting

Diospyros kaki L.f., Ebenaceae, have been used since centuries in traditional medicines in many Asian countries as it contains numerous flavonoids. Out of those plenty flavonoids, quercetin-3-O-β-D-glucopyranosyl-(1 → 6)-β-D-glucopyranoside (1) was found to be one of those active components of the plant. In the present study, we confirmed the non-toxicity behavior of 1 on RAW 264.7 macrophage cells and demonstrated its anti-inflammatory property. Our result indicates that 1 treatment significantly controlled the upregulation of the mitogen-activated protein kinase pathway in a dose-dependent manner in the Escherichia coli produced lipopolysaccharide induced infection background. In addition, its treatment significantly reduced the nitric oxide level, and down-regulated the protein expression of TNF-α, PGE2, IL-1β, IL-6, COX-2, and iNOS in lipopolysaccharide stimulated RAW 264.7 macrophage cells. Taken together, our results suggested that 1 efficiently modulates the MAPK signaling pathway and controlled the inflammatory event. Collectively, the result of the study confirmed the potential of 1 as a potent drug candidate and suggested its usage against the inflammatory diseases.