Isoflavone-Rich Extract of Trifolium resupinatum: Anti-obesity Attributes with In Silico Investigation of Its Constituents

• DOI: 10.1007/s43450-023-00501-8
• Publication Date: 2024-01-02T15:23:23Z
• Authors: Mona M. Marzouk; Alia Y. Ragheb; Elham M. Youssef; Nermin A. Ragab; Eman M. El-Taher; Ibrahim A. El Garf; Mona E. S. Kassem
• Keywords: Daidzin; Genistin; Phytochemical

Abstract Trifolium resupinatum L., Fabaceae, aqueous methanol leaf extract was selected to mitigate some obesity-associated risk factors validate the possibility of further developing herbal drugs. Chromatography and spectrophotometric techniques verified 14 phenolics, five which were first isolated from plant identified as 6''- O -acetyl ononin, genistin, daidzin, sissotrin, astragalin. Further phytochemical characterization performed via liquid chromatography-electrospray ionization-mass spectrometry assisted by a spectral similarity molecular network. In total, 81 metabolites tentatively annotated including 69 species-first dereplications. Two major isolates (formononetin pseudobaptigenin) along with investigated for an in vitro pancreatic lipase inhibition assay. They showed notable effects IC 50 values (µg/ml): 47.2 ± 1.1, 112.8 1.23, 471.32 0.8, respectively, incomparable orlistat (23.8 0.64). Preliminary vivo assay (25 mg/kg extract, daily, 8 weeks) displayed weight loss interest promising advancement serum triacylglycerides, total cholesterol, glucose levels. Molecular docking studies confirmed binding score formononetin pseudobaptigenin near active sites highlighted affinity other enzyme. Several passed Lipinski’s law drug-likeness test, whereas SwissADME radar that all constituents fall within acceptable bioavailability zone. Therefore, combination flavonoids, especially isoflavones, could be regarded drug-like agents protection against obesity-induced metabolic complaints. Graphical