Metabolic signatures of Arabidopsis thaliana abiotic stress responses elucidate patterns in stress priming, acclimation, and recovery

• DOI: 10.1007/s44154-022-00034-5
• Publication Date: 2022-02-15T00:03:58Z
• Authors: Yuan Xu; Dana M. Freund; Adrian D. Hegeman; Jerry D. Cohen
• Keywords: 2. Zero hunger; 0301 basic medicine; Original Paper; 03 medical and health sciences; 6. Clean water

Temperature, water, and light are three abiotic stress factors that have major influences on plant growth, development, reproduction. Plants can be primed by a prior mild to enhance their resistance future stress. We used an untargeted metabolomics approach examine Arabidopsis thaliana 11-day-old seedling's responses including heat (with without priming), cold water-deficit high-light before after 2-day-recovery period. Analysis of the physiological phenotypes showed seedlings with treatment resulted in reduction fresh weight, hypocotyl root length but remained viable. Several responsive metabolites were identified, confirmed reference standards, quantified, clustered. identified shared specific signatures for cold, heat, water-deficit, treatments. Central metabolism amino acid metabolism, sugar glycolysis, TCA cycle, GABA shunt, glutathione purine urea cycle found undergo changes fundamentally different, although some commonalities response different Large increases cysteine abundance decreases reduced observed following multiple treatments highlighting importance oxidative as general phenomenon fold low-turnover acids maltose demonstrate critical role protein starch autolysis early responses.