The transcription factor StMYB113 regulates light-induced greening by modulating steroidal glycoalkaloid biosynthesis in potatoes (Solanum tuberosum L.)

• DOI: 10.1007/s44281-023-00025-0
• Publication Date: 2024-03-20T22:45:01Z
• Authors: Shengxuan Liu; Yunxia Cheng; Xijuan Zhao; Enshuang Wang; Tiantian Liu; Huiling Zhang; Tengfei Liu; Song Botao
• Keywords: Glycoalkaloid; Solanum tuberosum

Abstract During harvesting, storage, transportation, and processing, potato ( Solanum tuberosum L . ) tubers undergo greening after exposure to light, leading the accumulation of toxic glycoside alkaloids, resulting in quality deterioration economic losses. However, underlying mechanisms are unclear. This study compared transcriptome proteome differences among four cultivars during light-induced process, identifying 3,751 unique proteins (high confidence; ≥91.7%). The levels enzymes involved steroidal glycoalkaloid biosynthesis varied cultivars. In addition, coexpression network analysis transcriptomic data identified transcription factor MYB113 (Soltu.DM.10G020780.1) as a potential positive regulator biosynthesis. dual-luciferase assay revealed that StMYB113 could bind promoters biosynthesis-related genes activate them. transgenic lines overexpressing L. Myb domain protein exhibited greater mRNA abundance these elevated glycoalkaloids. provided theoretical basis for exploring impact light on synthesis solanine potatoes.