Combining network pharmacology and experimental validation to investigate the role of preserved egg white in intervening in chronic atrophic gastritis

• DOI: 10.1007/s44403-025-00023-2
• Publication Date: 2025-04-14T23:02:11Z
• Authors: Jingying Sun; Yulin Wang; Yuanyuan Zhang; Yuxi Peng; Wei Liu; Xiaomeng Li; Zhaoxia Cai; Xi Huang; Youyou Lu; Long Sheng
• Keywords: Atrophic gastritis; White (mutation)

Abstract Chronic atrophic gastritis (CAG) is regarded as a transitional stage in the progression from to gastric tumors. Although preserved egg white (PEW) recognized for its anti-inflammatory properties, precise impact and mechanisms treating inflammation are not fully understood. This study employed an integrative approach, combining network pharmacology with experimental validation, elucidate therapeutic effects of PEW on CAG underlying mechanisms. Through pharmacology, potential targets associated signaling pathways were identified. The results indicated that peptides within involved intervention alongside 61 targets. KEGG enrichment analysis unveiled 99 pathways. Molecular docking revealed favorable binding properties between PEW’s main active compounds core model was established using N-Methyl-N´-nitro-N-nitrosoguanidine (MNNG) comprehensive method. Cellular experiments demonstrated significant reduction secretion IL-6 (41.73%), IL-8 (20.35%), TNF-α (31.49%)， while enhancing cell proliferation motility ( P < 0.05). Animal showed alignment rat tissue glands, increased pepsin activity, reduced inflammatory factors serum, regulation Bcl-2 NF-κB expression following treatment. These findings collectively highlight CAG, presenting promising alternative treatment approach.