Molecular characterization, expression patterns, and subcellular localization of a classical and a novel nonclassical MHC class I α molecules from Japanese eel Anguilla japonica

• DOI: 10.1016/j.aaf.2021.08.005
• Publication Date: 2021-09-17T07:40:35Z
• Authors: Jianjun Feng; Xinwei Peng; Peng Lin; Yilei Wang; Ziping Zhang; Yuankai Xu; Pengyun Chen; Jingying Wu
• Keywords: Homology

Major histocompatibility complex (MHC) molecules play a critical role in the immune response of vertebrate animals by presenting foreign antigens to T lymphocytes. In this study, we first cloned and identified classical novel nonclassical MHC I α from Japanese eel (Anguilla japonica), named as AjMHC I-UBA I-L, respectively. The full-length cDNA contains an open reading frame (ORF) 1047 bp encoding predicted protein 348 amino acids, while I-L 1089 encodes 362 acids. multiple alignment acid sequence showed that consist N-terminal superfamily domain within α1 α2 helices, IgC-MHC α3 domain, transmembrane region close C-terminal, which are similar other fish mammal species. Molecular polymorphism analysis eight different major alleles I-UBA, I-UBA*0101~1001, were six individuals. Furthermore, distinguishing signature L-lineage genes specific motif "HINMTL", including N-glycosylation site (NXS/T), was present sequences. Although three-dimensional structures human α, phylogenetic these two belong UBA gene U-lineage L-lineage, Quantitative real-time polymerase chain reaction (qRT-PCR) revealed highest expression found intestine level all examined tissues significantly higher than I-L. expressions liver, kidney spleen induced following injection with viral mimic poly I:C, LPS, Aeromonas hydrophila infection. vitro, transcripts liver cells enhanced treatment I:C or stimulation high concentration A. hydrophila. Subcellular localization under natural conditions, uniformly distributed cytoplasm, aggregated into spots flakes cytoplasm after LPS. These results collectively suggested important components possibly involved defense against bacterial Taken together, findings provide valuable insight function class system teleost.