GDNF facilitates the differentiation of ADSCs to Schwann cells and enhances nerve regeneration through GDNF/MTA1/Hes1 axis

• DOI: 10.1016/j.abb.2024.109893
• Publication Date: 2024-02-01T23:39:58Z
• Authors: Weixia Cai; Yang Liu; Ting Zhang; Peng Ji; Chenyang Tian; Jiaqi Liu; Zhao Zheng
• Keywords: Schwann cell; Nerve guidance conduit; HES1

Adipose tissue-derived stem cells (ADSCs) are a kind of with multi-directional differentiation potential, which mainly restore tissue repair function and promote cell regeneration. It can be directionally differentiated into Schwann-like to the peripheral nerve injury. Glial line-derived neurotrophic factor (GDNF) plays an important role in injury, but underlying mechanism remains unclear, seriously limits its further application.The study aimed identify molecular by overexpression glial line–derived facilitates ADSCs Schwann cells, enhancing regeneration after In vitro, overexpressing GDNF for 48 h exhibited changes their morphology, 80% having two or more prominences. Compared that ADSCs, GDNF-ADSCs increased expression marker S100, damage repair-related factors.ADSC normal culture ADSC were GDNF(GDNF-ADSCs) analysed using TMT-Based Proteomic Analysis revealed significantly higher MTA1 than control ADSCs. Hes1 was decreased silencing, along simultaneous decrease S100 factors. These findings indicate promotes induces factors accelerate repair.