Diversity and genetic characterization of Chlamydia isolated from Siamese crocodiles (Crocodylus siamensis)

• DOI: 10.1016/j.actatropica.2024.107183
• Publication Date: 2024-03-12T01:41:10Z
• Authors: Somjit Chaiwattan...; Metawee Thongdee; Nlin Arya; Weena Paungpin; Wanna Sirimanapong; Ladawan Sariya

Chlamydiosis, an infection caused by several Chlamydia species, has been reported in Nile, saltwater, and Siamese crocodiles. Despite its widespread reports various countries, including Thailand, genetic information on species remains limited. This study presents a whole-genome-based characterization of crocodile-isolated Chlamydia. The results showed that crocodile contained single circular chromosome with size 1.22–1.23 Mbp plasmid 7.7–8.0 kbp. A containing eight coding sequences (CDSs) was grouped β lineage. sequence had approximately 1,018–1,031 CDSs. Chlamydial factors involving virulence were documented terms the presence cytotoxins chromosomes analysis antimicrobial resistance genes genome revealed most common genes, comprising 26%–33% identified associated macrolide antibiotics, such as azithromycin, erythromycin, clarithromycin, loose matching (identities between 21.12% 74.65%). Phylogenetic analyses, encompassing assessments both whole proteome nine taxonomic markers, separated into three lineages (lineages I–III) high bootstrapping statistic support. Interestingly, isolate 12-01 differed genetically from others, suggesting it is new member findings indicate crocodiles are susceptible hosts to Chlamydia, more than one species. first employing highest number whole-genome data provides better insights pathogen genetics.