An interleukin-4-loaded bi-layer 3D printed scaffold promotes osteochondral regeneration

• DOI: 10.1016/j.actbio.2020.09.039
• Publication Date: 2020-09-29T23:13:35Z
• Authors: Lin Gong; Jun Li; Jingwei Zhang; Zongyou Pan; Yanshan Liu; Feifei Zhou; Yi Hong; Yejun Hu; Yuqing Gu; Hongwei Ouyang; Xiaohui Zou; Shufang Zhang
• Keywords: Polycaprolactone; Gelatin; Chondrogenesis

Multilayer scaffolds fabricated by 3D printing or other techniques have been used to repair osteochondral defects. However, it remains a challenge regenerate the articular cartilage and subchondral bone simultaneously with higher performance. In present study, we enhanced efficiency of defects developing bi-layer scaffold: an interleukin-4 (IL-4)-loaded radially oriented gelatin methacrylate (GelMA) scaffold printed digital light processing (DLP) in upper layer porous polycaprolactone hydroxyapatite (PCL-HA) fused deposition modeling (FDM) lower layer. An vitro test showed that both layers supported cell adhesion proliferation, as promoted osteogenic differentiation IL-4 relieved negative effects inflammation on murine chondrocytes, which were induced interleukin-1β (IL-1β) M1 macrophages. rabbit defect model, IL-4-loaded group obtained highest histological score (24 ± 2) compared nontreated (11 1) pure (16 groups after 16 weeks implantation, regeneration increased formation neocartilage neobone tissues. Thus, is attractive candidate for