Multiphoton imaging of melanoma 3D models with plasmonic nanocapsules

• DOI: 10.1016/j.actbio.2022.01.052
• Publication Date: 2022-01-31T00:47:10Z
• Authors: Paula Zamora-Perez; Can Xiao; Marcos Sanles-Sob...; Muriel Rovira-Esteva; José Javier Conesa; Vladimir Mulens-A...; Daniel Jaque; Pilar Rivera-Gil
• Keywords: Nanocapsules; Phototoxicity

We report the synthesis of plasmonic nanocapsules and cellular responses they induce in 3D melanoma models for their perspective use as a photothermal therapeutic agent. The wall is composed polyelectrolytes. inner part functionalized with discrete gold nanoislands. cavity contains fluorescent payload to show ability loading cargo. exhibit simultaneous two-photon luminescent, properties X-ray contrasting ability. average fluorescence lifetime (τ) measured FLIM (0.3 ns) maintained regardless intracellular environment, thus proving abilities bioimaging such spheroids complex architecture. Their multimodal imaging are exploited first time study tumorspheres exposed nanocapsules. Specifically, we studied uptake, toxicity, fate, generation reactive oxygen species, effect on levels hypoxia by using multi-photon confocal laser scanning microscopy. Because high attenuation atomic number nanostructure, imaged nanocapsule-cell interactions without processing sample. confirmed maintenance nanocapsules' geometry milieu no impairment ultrastructure. Furthermore, observed lack toxicity alteration or species levels. These results contribute development these exploitation future applications agents imaging-guided therapy. novelty work that our multimodal. They responsive multiphoton single-photon excitation. This allowed us interaction 2D structures specifically obtain information tumor cell parameters hypoxia, toxicity. will be further validated probes.