In vitro and in vivo assessment of squeeze-cast Mg-Zn-Ca-Mn alloys for biomedical applications

• DOI: 10.1016/j.actbio.2022.07.040
• Publication Date: 2022-07-30T06:44:28Z
• Authors: Dae Hyun Cho; Thomas Avey; Kyoung Hyup Nam; David Dean; Alan A. Luo
• Keywords: Biocompatible Materials; 02 engineering and technology; Rats; Corrosion; Rats, Sprague-Dawley; Zinc; X-Ray Diffraction; 0205 materials engineering; Absorbable Implants; Materials Testing; Alloys; Animals

Squeeze casting of biodegradable Mg-4Zn-0.5Ca-xMn (x = 0, 0.4 or 0.8 all in weight %) alloys is a process intended to improve mechanical properties (i.e., strength and ductility), corrosion performance (i.e., resistance), and biocompatibility (i.e., little to no cytotoxicity). In this study, we found that an increased Mn content causes the dendritic microstructure of squeeze-cast Mg-4Zn-0.5Ca-xMn to become more refined and uniform, while the volume fraction of the Ca2Mg6Zn3 phase simultaneously increases. Squeeze-cast Mg-4Zn-0.5Ca-0.8Mn presents the best yield strength, ultimate tensile strength, and elongation of the alloys tested. An electrochemical corrosion test in Hanks' solution at 36.5°C demonstrates that the corrosion resistance of squeeze-cast Mg-4Zn-0.5Ca-xMn alloys show improvement at higher Mn levels. Additionally, squeeze-cast Mg-4Zn-0.5Ca alloys containing Mn exhibit favorable biocompatibility, as evidenced by cell viability studies with MC3T3-E1 cells and a local lymph node assay test. Squeeze-cast alloy specimens implanted into the skull and spine of Sprague-Dawley rats for four weeks showed no serious cytotoxicity or foreign body response; however, swelling was observed in the implantation areas of Mn-free squeeze-cast Mg-4Zn-0.5Ca alloy, while no swelling was observed in rats implanted with Mn-containing Mg-4Zn-0.5Ca alloy. These findings indicate potential applications of biodegradable, Mn-containing, squeeze-cast Mg-4Zn-0.5Ca specimens in bone-reconstruction devices given their biocompatibility, mechanical properties, and degradation profiles. STATEMENT OF SIGNIFICANCE: Bioresorbable magnesium alloys have recently gained attention as viable biomaterials for skeletal reconstruction implants. Extensive research on biodegradable Mg alloy design, synthesis, and as-cast versus post-processed material properties useful for medical applications have been reported. The squeeze-casting technique used in this study can improve the mechanical properties (i.e., strengthening) and corrosive performance (reduced rate) of bioresorbable Mg-Zn-Ca-Mn alloys. Squeeze-casting of these alloys is also expected to improve specimen microstructure, near-net-shape manufacturing, and cost (i.e., reduced). This study provides an in vitro and in vivo assessment of squeeze-cast Mg-Zn-Ca-Mn alloys for biomedical applications.