Phase composition of calcium phosphate materials affects bone formation by modulating osteoclastogenesis

• DOI: 10.1016/j.actbio.2024.01.022
• Publication Date: 2024-01-23T18:03:04Z
• Authors: Paul Humbert; Carina Kampleitner; Julien De Lima; Meadhbh Á Brennan; Irene Lodoso-Torr...; Joanna Maria Sado...; Frédéric Blanchard; Cristina Canal; Maria-Pau Ginebra; Oskar Hoffmann; Pierre Layrolle
• Keywords: Bone remodeling; Biomaterial; Bone tissue

Human mesenchymal stromal cells (hMSCs) seeded on calcium phosphate (CaP) bioceramics are extensively explored in bone tissue engineering and have recently shown effective clinical outcomes. In previous pre-clinical studies, hMSCs-CaP-mediated formation was preceded by osteoclastogenesis at the implantation site. The current study evaluates to what extent phase composition of CaPs affects osteoclast response ultimately influence formation. To this end, four different CaP were used, hydroxyapatite (HA), β-tricalcium (β-TCP) two biphasic composites HA/β-TCP ratios 60/40 20/80 respectively, for vitro differentiation correlation with vivo All ceramics allowed from mouse human precursors, except pure HA, which significantly impaired their maturation. Ectopic alongside hMSCs subcutis sites nude mice revealed new 8 weeks all conditions relative amounts β-TCP > HA. Surprisingly, while essential osteoinduction, survival did not correlate By contrast, degree early (2 weeks) seemed define subsequent Together, our findings suggest that osteoclastic could be used as a predictive marker hMSC-CaP-based regeneration strengthens need understand underlying mechanisms future biomaterial development. combination (MSCs) materials has demonstrated its safety efficacy trials, despite insufficient understanding biological mechanisms. Osteoclasts previously suggested key mediators between inflammatory following Here we compared affinity osteoclasts various phosphate. We found formation, both stages vivo, correlates when implanted MSCs mice. MSC suggesting number or phenotype formed more important.