Activation of invasiveness of cervical carcinoma cells by angiotensin II

• DOI: 10.1016/j.ajog.2003.12.013
• Publication Date: 2004-05-29T15:05:44Z
• Authors: Fumitaka Kikkawa; Mika Mizuno; Kiyosumi Shibata; Hiroaki Kajiyama; Takanori Morita; Kazuhiko Ino; Seiji Nomura; Shigehiko Mizutani
• Keywords: Vascular Endothelial Growth Factor A; Angiotensin II; Uterine Cervical Neoplasms; Immunohistochemistry; Sensitivity and Specificity; 3. Good health; 03 medical and health sciences; Receptors, Vascular Endothelial Growth Factor; 0302 clinical medicine; Reference Values; Case-Control Studies; Biomarkers, Tumor; Carcinoma, Squamous Cell; Tumor Cells, Cultured; Humans; Female; Neoplasm Invasiveness; Cell Division; Probability

Angiotensin II recently has been reported to promote the growth of several kinds of cells. In this study, we investigated the effect of angiotensin II on cervical carcinoma cells.The expression of angiotensin II type I receptor was examined by immunohistochemistry in normal and neoplastic cervical tissues. Invasion assay was examined in Siha cells (cervical squamous cell carcinoma) and vascular endothelial growth factor levels were assayed with a vascular endothelial growth factor enzyme-linked immunosorbent assay kit.Mean staining intensity level was stronger in invasive carcinoma cells than in normal, dysplasia, and carcinoma in situ tissues. Angiotensin II induced the secretion of vascular endothelial growth factor from Siha cells. Furthermore, angiotensin II promoted the invasive potential of Siha cells. These effects were reversed by the addition of anti-human vascular endothelial growth factor antibody and candesartan (antagonist of angiotensin II type I receptor).Angiotensin II is involved in the progression of cervical carcinoma, because it induces the secretion of vascular endothelial growth factor through angiotensin II type I receptor, which results in the increased invasiveness of carcinoma cells.