MicroRNA 96 Is a Post-Transcriptional Suppressor of Anaplastic Lymphoma Kinase Expression

• DOI: 10.1016/j.ajpath.2012.01.008
• Publication Date: 2012-03-10T16:11:55Z
• Authors: Deeksha Vishwamitra; Yong Li; Desiree Wilson; Roxsan Manshouri; Choladda V. Curry; Bin Shi; Xi Ming Tang; Andrea M. Sheehan; Ignacio I. Wistuba; Ping Shi; Hesham M. Amin
• Keywords: Anaplastic large-cell lymphoma; ALK inhibitor

Anaplastic lymphoma kinase (ALK) constitutes a part of the oncogenic fusion proteins nucleophosmin-ALK and echinoderm microtubule-associated protein like 4-ALK, which are aberrantly expressed in subset T-cell anaplastic large-cell non-small-cell lung cancer, respectively. The expression mutated, constitutively active ALK also occurs neuroblastoma tumors. is believed to play an important role promoting tumor survival. Nevertheless, mechanisms underlying cancer cells not completely known. MicroRNA (miR) has been implicated regulation both oncogenes suppressor genes. We tested hypothesis that could be regulated by miR. Three Internet-based algorithms identified miR-96 potentially bind with 3'-untranslated region. Notably, levels were markedly decreased ALK-expressing cell lines primary human tumors compared their normal cellular tissue counterparts. Transfection different forms protein, without significant effects on mRNA. Furthermore, phosphorylation target proteins, including Akt, STAT3, JNK, type I insulin-like growth factor receptor, it down-regulated JunB. These associated reduced proliferation, colony formation, migration cells. data provide novel evidence decreases represent mechanism aberrant