Specific Lipopolysaccharide Serotypes Induce Differential Maternal and Neonatal Inflammatory Responses in a Murine Model of Preterm Labor

• DOI: 10.1016/j.ajpath.2015.05.015
• Publication Date: 2015-07-26T15:12:53Z
• Authors: Roberta Migale; Bronwen R. Herbert; Yun S. Lee; Lynne Sykes; Simon N. Waddington; Donald Peebles; Henrik Hagberg; Mark R. Johnson; Phillip R. Bennett; David A. MacIntyre
• Keywords: Chorioamnionitis

Intrauterine inflammation is recognized as a key mediator of both normal and preterm birth but also associated with neonatal neurological injury. Lipopolysaccharide (LPS) often used to stimulate inflammatory pathways in animal models infection/inflammation-induced labor; however, inconsistencies maternal responses LPS are frequently reported. We hypothesized that serotype-specific may account for portion these inconsistencies. Four different Escherichia coli serotypes (O111:B4, O55:B5, O127:B8, O128:B12) were administered CD1 mice via intrauterine injection at gestational day 16. Although control animals delivered term 60 ± 15 hours postinjection (p.i.), those O111:B4 7 2 p.i., O55:B5 10 3 O127:B8 16 O128:B12 17 p.i. (means SD). A correlation between the onset labor myometrial activation transcription factor, activator protein 1, not NF-κB was observed. Specific induced differential downstream contractile myometrium pup brain. Our findings demonstrate functional disparity pathway response differing serotypes. Selective use represent useful tool targeting specific mechanisms models.