Pulsed Electric Field for protein release of the microalgae Chlorella vulgaris and Neochloris oleoabundans

• DOI: 10.1016/j.algal.2017.03.024
• Publication Date: 2017-04-01T20:02:18Z
• Authors: G.P. 't Lam; P.R. Postma; D.A. Fernandes; R.A.H. Timmermans; M.H. Vermuë; M.J. Barbosa; M.H.M. Eppink; R.H. Wijffels; G. Olivieri
• Keywords: Chlorella vulgaris

Pulsed Electric Field (PEF) is currently discussed as promising technology for mild and scalable cell disintegration of microalgae. In this study Chlorella vulgaris Neochloris oleoabundans have been subjected to batch continuous PEF treatments under a wide range operating conditions (1–40 pulses, 0.05–5 ms 7.5–30 kV cm− 1, 0.05–150 kWh kgDW− 1). many cases after treatment, both algal species show release ions, which indicates that treatment resulted in permeabilization the cell. However, electroporation effect was not sufficient substantially intracellular proteins. Even at severe energy input (10 100 times higher than bead milling) only up 13% proteins released from cells comparison 45–50% milling.