Expression and production of thermophilic alginate lyases in Bacillus and direct application of culture supernatant for seaweed saccharification

• DOI: 10.1016/j.algal.2021.102512
• Publication Date: 2021-10-12T09:44:47Z
• Authors: Nanna Rhein-Knudsen; Chengran Guan; Geir Mathiesen; Svein Jarle Horn
• Keywords: 0303 health sciences; 03 medical and health sciences

Brown seaweeds are rich in carbohydrates and may be used as a source of fermentable sugars. Saccharification the seaweed biomass can carried out enzymatically by combination cellulases alginate lyases. In this study, thermotolerant exo- endo lyases were cloned expressed Bacillus subtilis. The secreted to culture supernatant directly together with commercial cellulase preparation saccharify Saccharina latissima biomass. results showed that strategy using supernatants worked very well, releasing glucose, mannitol, uronic acids. ratio between endo-acting proved important for saccharification yield, under optimal reaction conditions use containing improved final glucose concentration 73%, when compared only applying cellulases. This direct shows enzyme purification steps not needed, saving processing costs points possibility relatively simple on-site production biorefining.