Influence of previous plane of nutrition on molecular mechanisms regulating the expression of urea and water metabolism related genes in the rumen and kidney of finishing crossbred Angus steers

• DOI: 10.1016/j.aninu.2023.12.011
• Publication Date: 2024-03-16T05:35:13Z
• Authors: Aghata E. Moreira...; Arturo Macias Franco; Bradley S. Ferguson; Mozart A. Fonseca
• Keywords: Aquaporin 2

This study aimed to understand how molecular mechanisms controlling water and urea metabolism at the finishing phase can be affected by previous plane of nutrition crossbred Angus beef steers. Twenty-four (n = 24) animals were randomly distributed into either a moderate (MP) or high during background for 85 d. Animals then blocked their moved onto 105-d in 2 × factorial arrangement. The forage-finished group received only high-quality alfalfa hay, whereas grain-fed grain diet (80% whole corn 20% hay). By end phase, harvested tissue samples from rumen kidney collected. Changes gene expression aquaporins (AQP) -2, -3, -4, -7, ATP1A1, ATP1B1, SGK1, CLIC1 (kidney rumen), UT-A1 only) UT-B (rumen only), assayed via real-time qPCR; 18S rRNA was used as an endogenous control. One-way ANOVA followed Tukey's post hoc analysis conducted. When MP, forage-finishing increased relative abundance AQP3 (P < 0.05), AQP7 ATP1B1 SGK1 0.05) when compared animals. In rumen, MP group, differentially expressed both treatments 0.01), with steers having highest AQP7. For had tendency presenting higher on 0.075). Overall, these results suggest that impact genes associated namely AQP3, AQP7, kidney, rumen. greatest observed changes investigated reflective backgrounded plane.