Effect of low density lipoprotein on DNA integrity of freezing–thawing boar sperm by neutral comet assay

• DOI: 10.1016/j.anireprosci.2006.08.022
• Publication Date: 2006-10-12T11:29:39Z
• Authors: Zhong-Liang Jiang; Qing-Wang Li; Wen-Ye Li; Jian-Hong Hu; Hong-Wei Zhao; Shu-Shan Zhang
• Keywords: BOAR; Comet Assay; Diluent

A modified protocol of neutral comet assay was utilized to assess the effect of low density lipoprotein (LDL) on the DNA integrity of boar freezing-thawing semen. The results demonstrated that the method was high sensitive and easier manipulation and LDL significantly protected sperm DNA integrity (p<0.05) from the damage caused by cryopreservation except TD at the concentration of 6%, 7% and TM at 6%, the optimal LDL concentration in diluents was 9%. Moreover, LDL showed better protection in 0.25 ml than in 0.5 ml types of straw (p<0.05) and no difference was observed in the same volume straw at the concentration of 9% and 10%. It was just the same for LDL effect on boar sperm DNA in cryopreservation 0 day and 30 days (p>0.05).