In vitro production of sex preselected cattle embryos using a monoclonal antibody raised against bull sperm epitopes

• DOI: 10.1016/j.anireprosci.2018.11.006
• Publication Date: 2018-11-16T16:59:08Z
• Authors: M.M.R. Chowdhury; Xu Lianguang; Rami Kong; Bun-Young Park; Ayman Mesalam; Myeong-Don Joo; Fahmida Afrin; Jong-In Jin; Hyun-Tae Lim; Il-Keun Kong
• Keywords: Male; 0301 basic medicine; X Chromosome; Antibodies, Monoclonal; Embryonic Development; Cell Separation; Fertilization in Vitro; Embryo Transfer; Embryo, Mammalian; Spermatozoa; 3. Good health; Embryo Culture Techniques; Epitopes; 03 medical and health sciences; Y Chromosome; Oocytes; Animals; Cattle; Female; Sex Preselection

Sex preselection has always generated great interest among livestock producers. Among the prevalent sperm sorting methods, there is much evidence that sex a negative effect on quality with an altered pattern of motility, ultimately reducing period cell viability. In this study, we have established new approach for preselected embryo production by using WholeMom®; monoclonal antibody developed against bull epitopes simple and easy separation X- Y-sperm. There were no significant differences (P > 0.05) in percentage presumptive zygotes between control X-sperm sorted group, but was difference early cleaving embryos being 81.2 ± 1.4%, 78.3 1.0%, 66.7 1.1% control, sorted, Y-sperm groups, respectively. Similarly, to blastocyst stage (Day 7) also greater < group compared 34.8 32.1 0.8%, 23.7 1.0% Furthermore, B-SRY F2 R2 gene expression data indicated detection accuracy 81.0% female 72.5% male produced vitro. conclusion, cattle vitro derived pre-selected sexed semen subsequent transfer can facilitate mass individuals are genetically superior.