Virucidal and antiviral activity of astodrimer sodium against SARS-CoV-2 in vitro

• DOI: 10.1016/j.antiviral.2021.105089
• Publication Date: 2021-05-16T21:41:22Z
• Authors: Jeremy R.A. Paull; Graham P. Heery; Michael D. Bobardt; Alex Castellarnau; Carolyn A. Luscombe; Jacinth K. Fairley; Philippe A. Gallay
• Keywords: Vero cell; Cytopathic effect; Infectivity; Infectious dose

An effective response to the ongoing coronavirus disease (COVID-19) pandemic caused by severe acute respiratory syndrome 2 (SARS-CoV-2) will involve a range of complementary preventive modalities. The current studies were conducted evaluate in vitro SARS-CoV-2 antiviral and virucidal (irreversible) activity astodrimer sodium, dendrimer with broad spectrum antimicrobial activity, including against enveloped viruses vivo models, that is marketed for antibacterial applications. We report sodium inhibits replication Vero E6 Calu-3 cells, 50% concentrations (EC50) i) reducing virus-induced cytopathic effect 0.002–0.012 mg/mL ii) infectious virus release plaque assay 0.019–0.032 cells 0.030–0.037 cells. selectivity index (SI) these assays was as high 2197. Astodrimer also virucidal, irreversibly infectivity >99.9% (>3 log10) within 1 min exposure, up >99.999% (>5 shown at 10–30 cell lines. inhibited infection primary human airway epithelial line. data similar all investigations consistent potent being due irreversible inhibition virus-host interactions, previously demonstrated other viruses. Further confirm if binds spike protein physically blocks initial attachment host cell. Given effectiveness significantly SI, warrants further investigation potential topically administered agent therapeutic