A polyphenol-assisted IL-10 mRNA delivery system for ulcerative colitis

• DOI: 10.1016/j.apsb.2022.03.025
• Publication Date: 2022-04-02T23:48:01Z
• Authors: Zhejie Chen; Wei Hao; Caifang Gao; Yangyang Zhou; Chen Zhang; Jinming Zhang; Ruibing Wang; Yitao Wang; Shengpeng Wang
• Keywords: Ex vivo

With the development of synthesis technology, modified messenger RNA (mRNA) has emerged as a novel category therapeutic agents for broad diseases. However, effective intracellular delivery mRNA remains challenging, especially its sensitivity to enzymatic degradation. Here, we propose polyphenol-assisted handy strategy efficient in vivo IL-10 mRNA. binds polyphenol ellagic acid through supramolecular binding yield negatively charged core, followed by complexing with linear polyetherimide and coating bilirubin-modified hyaluronic obtain layer-by-layer nanostructure. The nanostructure specifically up-regulated level IL-10, effectively inhibited expression inflammatory factors, promoted mucosal repair, protected colonic epithelial cells against apoptosis, exerted potent efficacy dextran sulfate sodium salt-induced acute chronic murine models colitis. designed system without systemic toxicity potential facilitate promising platform ulcerative colitis treatment.